Hamas’ de facto government head Essam Addalees and internal security chief Mahmoud Abu Watfa were among those killed by Israeli strikes on Tuesday, in addition to several other officials.
Palestinian health officials said at least 400 people, more than half of them women and children, were killed on Tuesday.
Palestinian medics said an Israeli plane bombed a house in Rafah, wounding several people.
Hamas has accused Israel of breaking the terms of the January ceasefire agreement by refusing to begin negotiations for an end to the war and a withdrawal of its troops from Gaza. But Hamas has said it is still willing to negotiate and was studying “bridging” proposals from US President Donald Trump’s special envoy, Steve Witkoff.
The return to the air strikes and ground operations that have devastated Gaza has drawn calls for a ceasefire from Arab and European countries. Britain, France and Germany issued a joint statement calling on Israel to restore access for humanitarian aid.
Israel has blocked the entry of goods into Gaza and Netanyahu’s foreign policy adviser, Ophir Falk, accused Hamas of taking aid for its own use, a charge Hamas has previously denied.
Israel launched its campaign in Gaza after a devastating Hamas attack on Israeli communities around the Gaza Strip on Oct 7, 2023 that killed some 1,200 people, according to an Israeli tally, and saw 251 abducted as hostages.
The Israeli campaign has killed more than 49,000 Palestinians, according to Palestinian health authorities, and devastated much of the coastal enclave, leaving hundreds of thousands of people in tents and makeshift shelters.
